Antimutagenic and antioxidant effects of a South African traditional formulation used as an immune booster

Authors

  • Mlungisi Ngcobo Traditional Medicine Laboratory, School of Nursing and Public Health, College of Health Sciences, University of KwaZulu Natal, Durban, South Africa
  • Nceba Gqaleni Department of Public Management and Economics, Durban University of Technology, Durban, South Africa
  • Victor Ndlovu Makhonya Natural Health Products (Pty) Ltd, Durban, South Africa

DOI:

https://doi.org/10.17159/sajs.2016/20150152

Keywords:

traditional medicines, regulations, genotoxicity, antioxidants, immune cells

Abstract

The traditional medicines sector in South Africa is still largely unregulated despite legislation aimed at regulating the practice being in place. The HIV and AIDS epidemic has fuelled demand for traditional medicines, with many patients consulting traditional health practitioners who offer different treatments, including herbal immune boosters. This study investigated the mutagenic and antioxidant effects of the widely sold herbal immune booster, uMakhonya®. The Ames test was used for analysis of the genototoxic effects while the adenosine triphosphate (ATP) assay was used to evaluate cell cytotoxicity in peripheral blood mononuclear cells (PBMCs) and THP-1 monocytes. To evaluate the antioxidant effects the malondialdehyde (MDA) quantification, the nitric oxide and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assays were used. UMakhonya® doses of up to 5000 μg/mL were not genotoxic in the Ames test. UMakhonya® was shown to induce dose-dependent cytotoxicity in both PBMCs and THP-1 cells with doses ranging from 500 μg/ mL to 1000 μg/mL, showing significant (p<0.05) toxicity. UMakhonya® was able to significantly (p<0.05) reduce nitrite radicals at 100 μg/mL while lower doses were not effective when compared to samples stimulated by lipopolysaccharide only. Non-cytotoxic doses of uMakhonya® showed significant (p<0.05) lipid peroxide scavenging ability in supernatants while this scavenging ability was considerably reduced intracellularly. In the DPPH assay, when both uMakhonya® and ascorbic acid were reconstituted in buffered saline, the traditional herbal remedy showed better radical scavenging abilities. Therefore further studies on the genotoxicity of uMakhonya®, when metabolically activated, and its antioxidant effects in in-vivo models are warranted.

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Published

2016-03-30

How to Cite

Ngcobo, M., Gqaleni, N., & Ndlovu, V. (2016). Antimutagenic and antioxidant effects of a South African traditional formulation used as an immune booster. South African Journal of Science, 112(3/4), 7. https://doi.org/10.17159/sajs.2016/20150152

Issue

Section

Research Article